Hi, I am recently analyzing the full length 16S rRNA amplicon sequenicng by QIIME2 (version 2023.07). I have encountered an error message during the denosing step.
Below is the commond that I am using for the denonosing:
qiime dada2 denoise-ccs --i-demultiplexed-seqs enrichment-css-demux.qza
--p-min-len 1000 --p-max-len 1600
--p-front AGRGTTYGATYMTGGCTCAG --p-adapter RGYTACCTTGTTACGACTT
After running this, an error message just pops up:
Plugin error from dada2:
No reads passed the filter. trunc_len (0) may be longer than read lengths, or other arguments (such as max_ee or trunc_q) may be preventing reads from passing the filter.
I wonder whether anyone encountered the same problem and could you please let me know how to fix this?
I attached the sequence quality and sequence number in each sample after importing into QIIME2
Thank you very much for your help!