# DADA2 and Deblur outputs are extremely different

**URL:** https://forum.qiime2.org/t/dada2-and-deblur-outputs-are-extremely-different/23574
**Category:** User Support
**Tags:** dada2, deblur
**Created:** [July 6, 2022, 6:14pm UTC](https://forum.qiime2.org/t/dada2-and-deblur-outputs-are-extremely-different/23574 "2022-07-06T18:14:33Z")
**Posts on this page:** 1
**Showing post:** 9

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### Author: ![SoilRotifer](https://forum.qiime2.org/user_avatar/forum.qiime2.org/soilrotifer/32/21071_2.png) [@SoilRotifer](https://forum.qiime2.org/u/SoilRotifer)
#### Post date: [July 8, 2022, 8:22pm UTC](https://forum.qiime2.org/t/dada2-and-deblur-outputs-are-extremely-different/23574/9 "2022-07-08T20:22:26Z")

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> [@Stephan\_Bitterwolf](#):
>
> Perhaps there is a way for me to add the missing sequence to SILVA? The sequence can be found on GenBank

Yes there is. You should be able to follow one of these tutorials:

> [@Using RESCRIPt to compile sequence databases and taxonomy classifiers from NCBI Genbank](https://forum.qiime2.org/t/using-rescript-to-compile-sequence-databases-and-taxonomy-classifiers-from-ncbi-genbank/15947):
>
> This tutorial will describe how to create custom reference databases from NCBI Genbank using [RESCRIPt](https://github.com/bokulich-lab/RESCRIPt). Read more about RESCRIPt here: [Processing, filtering, and evaluating the SILVA database (and other reference sequence data) with RESCRIPt - #7](https://forum.qiime2.org/t/processing-filtering-and-evaluating-reference-sequence-data-with-rescript/15494/7) If using NCBI Genbank data, please be aware of the [NCBI disclaimer and copyright notice](https://www.ncbi.nlm.nih.gov/home/about/policies/). Citation: If you use RESCRIPt or any RESCRIPt-processed data in your research, please cite the following pre-print: Michael S Robeson II, Devon R O'Rourke, Benj…

From here you should be able to run `qiime feature-table merge-taxa...` and `qiime feature-table merge-seqs ...` to combine the GenBank and SILVA data.

Note that, prior to merging, there is a chance you'd have to run `qiime rescript edit-taxonomy ...` on the fetched NCBI taxonomy data to change the `k __` prefixes to `d__ `, so that it matches SILVA. Assuming you are downloading the standard 7 ranks (kpcofgs). Otherwise you'll have to do more editing.

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