# Cutadapt not trimming 454 reads

**URL:** https://forum.qiime2.org/t/cutadapt-not-trimming-454-reads/20247
**Category:** User Support
**Tags:** 454, cutadapt, rescript
**Created:** [July 20, 2021, 12:39pm UTC](https://forum.qiime2.org/t/cutadapt-not-trimming-454-reads/20247 "2021-07-20T12:39:44Z")
**Posts on this page:** 1
**Showing post:** 4

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### Author: ![Natali\_Hernandez](https://forum.qiime2.org/user_avatar/forum.qiime2.org/natali_hernandez/32/8306_2.png) [@Natali\_Hernandez](https://forum.qiime2.org/u/Natali_Hernandez)
#### Post date: [July 21, 2021, 11:58am UTC](https://forum.qiime2.org/t/cutadapt-not-trimming-454-reads/20247/4 "2021-07-21T11:58:20Z")

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Hi Luca,

Many thanks for the suggestion, I've tried and the reads are shorter but not quite there yet!

 ![image](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/2X/2/26f02a132fb9e765308dbfba82683f9c96350e02.png) .

This is m first go with 454 sequences, so I am not sure if this is normal. I think I am looking at something similar to this [one](https://forum.qiime2.org/t/importing-two-directions-454-seq-data-for-denoise-pyro/17348/3), as suggested there, I'll try RESCRIPt, I'm just unsure about --i-reference-sequences reference-sequences.qza , can this be any other sequences, i.e. not 454 but Illumina?

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