Many thanks to your answers.
In fact, the I have tried this commend using 1 thread, 4 threads, 10 threads. They were all be killed
I will try using 1 thread again, and also try using --p-parttree or fragment-insertion.
By the way, I feel uncertain about the step of cutadapt trim-paired
- I found the seqs of the rep-seqs still containing the reverse primers. Why…
The used code was:
qiime cutadapt trim-paired --p-cores 20 --i-demultiplexed-sequences pre_5/demultiplexed-seqs.qza --p-anywhere-f CCTACGGGNGGCWGCAG --p-anywhere-r GGACTACHVGGGTATCTAATCC --o-trimmed-sequences pre_5/trimmed-seqs.qza --quiet
- In QIIME1, there is a parameter in split_barcode to adjust the sequence orientation according to the barcode. Is the QIIME2 has the same function so that the primers can be correctly recognized and trimmed??
Oh, I am really helpless…