Hello all, I have fungal data that I have assigned taxonomy to using the UNITE database. The assignments are pretty good down to the genus level for the most prevalent ASVs, but some of the most frequent ASVs have unfortunately been assigned to unidentified genera (eg, k__Fungi;p__Basidiomycota;c__Malasseziomycetes;o__Malasseziales;f__Malasseziaceae;g__unidentified).
Thus, I am hoping to manually assign taxonomy to these sequences using BLAST. My question is the following: after assigning taxonomy to these sequences of interest using BLAST, where in the QIIME pipeline analysis process could I plug in these adjustments (eg, to repseqs.qza, to taxonomy.qza). Is this even something that I can do in QIIME? If not, any ideas on how I would do this?
Thanks so much!