# Create barcode file

**URL:** https://forum.qiime2.org/t/create-barcode-file/3285
**Category:** User Support
**Tags:** import
**Created:** [March 2, 2018, 3:36pm UTC](https://forum.qiime2.org/t/create-barcode-file/3285 "2018-03-02T15:36:52Z")
**Posts on this page:** 1
**Showing post:** 3

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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [March 2, 2018, 4:36pm UTC](https://forum.qiime2.org/t/create-barcode-file/3285/3 "2018-03-02T16:36:30Z")

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Hi @cecibe,  
Where your barcodes are is all going to depend on how you constructed your sequencing library. Your 2 fastqs are probably the forward/reverse reads, and the barcodes may be in-line in the sequences or in the header lines — it is impossible to know without getting that information from the source (or you could check the first N nucleotides on each sequence to see if these match the barcodes in your sample metadata file). Check with the sequencing center, lab, or whoever did the library prep/sequencing, that's the surest way to know.

Once you figure out where the barcodes are, I can point you in the direction of the correct method for importing/demultiplexing.

Good luck!

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