Covertion zOTUs files from Usearch11 to Qiime2


I have some problems trying to convert some zOTUs files I obtained with a Usearch11 protocol to Qiime2 format. I want to do this to compare some results I got with a Qiime2 pipeline. I aim to know the differences between ASVs and zOTUs in my analysis. As a student, I would be grateful if anyone could help me with this task.

Hi @Rodrigo,

Much of what you'll need can be found in the Import data tutorial.

Specifically, if you have a biom formated version of the zOTU table output by usearch, you can import this via this approach.

Otherwise if you have a tsv file, you'll need to convert this to a biom format using one of the commands outlined here. Likely this one, which be run from within the qiime environment.:

biom convert -i table.txt -o table.from_txt_hdf5.biom --table-type="OTU table" --to-hdf5

Once you do this, you can then run the above linked import command.

Your FASTA sequences can be imported like this.