# converting pacbio

**URL:** https://forum.qiime2.org/t/converting-pacbio/30190
**Category:** Ideas and Suggestions
**Created:** [May 13, 2024, 3:45pm UTC](https://forum.qiime2.org/t/converting-pacbio/30190 "2024-05-13T15:45:02Z")
**Posts on this page:** 1
**Showing post:** 2

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### Author: ![colinbrislawn](https://forum.qiime2.org/user_avatar/forum.qiime2.org/colinbrislawn/32/6221_2.png) [@colinbrislawn](https://forum.qiime2.org/u/colinbrislawn)
#### Post date: [May 13, 2024, 4:47pm UTC](https://forum.qiime2.org/t/converting-pacbio/30190/2 "2024-05-13T16:47:54Z")

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Hi Alexander,

Sure, ya' can. The challenge will be that the Pacbio reads will be longer, so you have different regions.

Most (all?) amplicon analysis pipelines are predicated on sequencing the _same_ region, so this is a huge challenge!

Try [searching the forums for "multiple regions"](https://forum.qiime2.org/search?q=multiple%20regions) to find threads [like this](https://forum.qiime2.org/t/using-multiple-16s-variable-regions-for-analysis/28833) that gives some practical places to start.

I would simply treat these as two different data sets.

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