connect error of q2-clawback

[Nicholas_Bokulich]

Hi @RayLin ,
This looks like an issue connecting to Qiita. I just checked the Qiita website and they are updating the system today... so I suspect that is the cause.

You could probably try again tomorrow (or whenever the system update is complete).

@RayLin

If it’s a connection error, can I download the empo file from other site?

Sure! clawback can take any taxonomy data as input. Check out the " Assembling More Exotic Weights" section of this tutorial. The first step uses redbiom to get a biom table of samples x ASVs, but this could be replaced by other sources, e.g., grabbing data from the Earth Microbiome Project or another source.

Using q2-clawback to assemble taxonomic weights

Note: This guide assumes you have installed QIIME 2 using one of the procedures in the install documents. This tutorial extends the q2-feature-classifier tutorial to show how you can improve taxonomic classification accuracy by assembling ecologically informed taxonomic weights for your samples using q2-clawback. This approach is described in this article: https://doi.org/10.1038/s41467-019-12669-6 We will retrain the naive Bayes classifier from the q2-feature-classifier to classify the ski…

Good luck!