# Clustering ASVs into OTUs

**URL:** https://forum.qiime2.org/t/clustering-asvs-into-otus/28781
**Category:** User Support
**Tags:** dada2, vsearch
**Created:** [January 3, 2024, 2:56pm UTC](https://forum.qiime2.org/t/clustering-asvs-into-otus/28781 "2024-01-03T14:56:21Z")
**Posts on this page:** 6
**Page:** 1

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### Author: ![Vaishnavi\_Dixit](https://forum.qiime2.org/user_avatar/forum.qiime2.org/vaishnavi_dixit/32/17483_2.png) [@Vaishnavi\_Dixit](https://forum.qiime2.org/u/Vaishnavi_Dixit)
#### Post date: [January 3, 2024, 2:56pm UTC](https://forum.qiime2.org/t/clustering-asvs-into-otus/28781/1 "2024-01-03T14:56:21Z")

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Hi I am trying to cluster my ASVs into OTUs by following this Qiime Tutorial  
[https://docs.qiime2.org/2023.9/tutorials/otu-clustering/](https://docs.qiime2.org/2023.9/tutorials/otu-clustering/)  
I have tried adding my fasta file downloaded from rep-seq but I am getting this error

(1/1) Invalid value for '--i-sequences': Expected an artifact of at least  
type SampleData[Sequences] | SampleData[SequencesWithQuality] |  
SampleData[JoinedSequencesWithQuality]. An artifact of type  
FeatureData[AlignedSequence] was provided.

Further I am not able to convert fasta file in fna for initially converting in into qza

Please assist me with this

Thanks

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### Author: ![cherman2](https://forum.qiime2.org/user_avatar/forum.qiime2.org/cherman2/32/17129_2.png) [@cherman2](https://forum.qiime2.org/u/cherman2)
#### Post date: [January 3, 2024, 5:00pm UTC](https://forum.qiime2.org/t/clustering-asvs-into-otus/28781/2 "2024-01-03T17:00:46Z")

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### Author: ![cherman2](https://forum.qiime2.org/user_avatar/forum.qiime2.org/cherman2/32/17129_2.png) [@cherman2](https://forum.qiime2.org/u/cherman2)
#### Post date: [January 3, 2024, 6:22pm UTC](https://forum.qiime2.org/t/clustering-asvs-into-otus/28781/3 "2024-01-03T18:22:43Z")

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Hi @Vaishnavi_Dixit  
Welcome to the :qiime2: forum!  
Your issue is here:

> [@Vaishnavi\_Dixit](#):
>
> An artifact of type  
> FeatureData[AlignedSequence] was provided.

You are providing aligned sequences and your command doesn't allow aligned sequences. Do you have any of these types: SampleData[Sequences] | SampleData[SequencesWithQuality] |  
SampleData[JoinedSequencesWithQuality]?

> [@Vaishnavi\_Dixit](#):
>
> Further I am not able to convert fasta file in fna for initially converting in into qza

Can you provide more info? What command are you running and what does your data look like?

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### Author: ![cherman2](https://forum.qiime2.org/user_avatar/forum.qiime2.org/cherman2/32/17129_2.png) [@cherman2](https://forum.qiime2.org/u/cherman2)
#### Post date: [January 3, 2024, 6:22pm UTC](https://forum.qiime2.org/t/clustering-asvs-into-otus/28781/4 "2024-01-03T18:22:47Z")

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### Author: ![Vaishnavi\_Dixit](https://forum.qiime2.org/user_avatar/forum.qiime2.org/vaishnavi_dixit/32/17483_2.png) [@Vaishnavi\_Dixit](https://forum.qiime2.org/u/Vaishnavi_Dixit)
#### Post date: [January 5, 2024, 6:22am UTC](https://forum.qiime2.org/t/clustering-asvs-into-otus/28781/5 "2024-01-05T06:22:19Z")

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Hey, Thanks for your help.

I used the code available for Denovo clustering and directly provided a feature table and sequence as input and adjusted the similarity percentage.

Finally, it worked out.

Thanks so much for your input.

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [February 5, 2024, 12:23pm UTC](https://forum.qiime2.org/t/clustering-asvs-into-otus/28781/6 "2024-02-05T12:23:18Z")

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