Good Evening Everyone,
I hope everyone is doing well and are healthy and safe. I am very new to qiime2 and am attempting to analyze my data for the first time without a tutorial.
I am currently on the step in that I am trying to denoise my data (which is paired end sequences) using qiime dada2 denoise-paired. At this time I am trying to select my trim-left-r and trim-left-f values however I am having a little trouble in selecting them.
As a result, if someone could please help me in selecting these two values (trim-left-r and trim-left-f values) it would be greatly appreciated. I initially thought to select 6 for each however upon thinking I notice that the black bars start at 120 for reverse and ~96 for the forward. Therefore which one should i select?
Thank you very much and I hope everyone has a wonderful night