# Can't import SILVA 132 .fna file into QIIME2

**URL:** https://forum.qiime2.org/t/cant-import-silva-132-fna-file-into-qiime2/8360
**Category:** Silva
**Tags:** silva
**Created:** [February 14, 2019, 12:18am UTC](https://forum.qiime2.org/t/cant-import-silva-132-fna-file-into-qiime2/8360 "2019-02-14T00:18:38Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![Sam\_Degregori](https://forum.qiime2.org/user_avatar/forum.qiime2.org/sam_degregori/32/1350_2.png) [@Sam\_Degregori](https://forum.qiime2.org/u/Sam_Degregori)
#### Post date: [February 14, 2019, 12:18am UTC](https://forum.qiime2.org/t/cant-import-silva-132-fna-file-into-qiime2/8360/1 "2019-02-14T00:18:38Z")

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Hi all,

I am trying to train my own classifier on QIIME2 with the SILVA database but ran into an importing issue. I tried importing silva\_132\_99\_16S.fna from the SILVA database into qiime and I tried the following code which I got from [here](https://forum.qiime2.org/t/seqs-fna-is-not-a-n-qiime1demuxformat-file/2450)

#ran this first just to make sure all letters were capitalized as recommended in other post  
cat silva132\_99.fna | tr 'acgt' 'ACGT' \> seqs.fna

qiime tools import   
--input-path seqs.fna   
--output-path seqs.qza   
--type SampleData[Sequences]

And I get the following error: seqs.fna is not a(n) QIIME1DemuxFormat file

I also tried adding in the --input-format QIIME1DemuxFormat with no avail.  
I also tried this on the 16S only fna file from the SILVA 132 folder as well. I got the folder from [here](https://www.arb-silva.de/download/archive/qiime/).

Any clues as to why this might be happening? I may simply resort to using your pre-trained classifier that you have. Is there any knowledge as to how much effectiveness you lose when using a pre-trained classifier versus training with your own sequences?

Thanks for the help!

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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [February 14, 2019, 12:22am UTC](https://forum.qiime2.org/t/cant-import-silva-132-fna-file-into-qiime2/8360/2 "2019-02-14T00:22:32Z")

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> [@Sam\_Degregori](#):
>
> –type SampleData[Sequences]

The problem is that this is not sample data. Use `FeatureData[Sequence]` instead.

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [March 17, 2019, 6:22am UTC](https://forum.qiime2.org/t/cant-import-silva-132-fna-file-into-qiime2/8360/3 "2019-03-17T06:22:34Z")

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