I'm trying to assign taxonomy to my 18S fungal sequences, and I've tried the pre-built qiime classifier for SILVA with no success. I wanted to download this database from MIDORI: Download/GenBank256_2023-06-21/QIIME/uniq/MIDORI2_UNIQ_NUC_GB256_srRNA_QIIME.fasta.gz
From their website: Download
because it's already preformatted for qiime. I unzipped the file and tried importing it into qiime using these commands, and got the following error.
gzip -d MIDORI2_UNIQ_NUC_GB256_srRNA_QIIME.fasta.gz qiime tools import --input-path MIDORI2_UNIQ_NUC_GB256_srRNA_QIIME.fasta --output-pat h MIDORI_UNIQUE-18S.seq.qza --type ‘FeatureData[Sequence]’ Error: Detected invalid character in: ‘FeatureData[Sequence]’ Verify the correct quotes or dashes (ASCII) are being used.
Has anyone encountered this error before? I can't see anything weird in the file other than some sequence headers having extra '<' or '>' symbols. Thanks for any help!