Cannot filter unassigned taxa: k__Bacteria;__;__;__;__;__;__

Hi @nvtravan,
In the taxonomic assignment, those underscores are not present (they only appear when you visualize these data, e.g., as a barplot or collapse a feature table on taxonomy).

See this post

So in your case you want something like this:

qiime feature-table filter-features \
  --i-table table.qza \
  --m-metadata-file taxonomy.qza \
  --p-where "Taxon='k__Bacteria' \
  --o-filtered-table filtered-table.qza

I hope that helps! Let me know if you’re still having trouble.