# can someone help me to decide in denoising this paired end sequence
**URL:** https://forum.qiime2.org/t/can-someone-help-me-to-decide-in-denoising-this-paired-end-sequence/29054
**Category:** User Support
**Tags:** dada2, feature-table
**Created:** [January 26, 2024, 2:41pm UTC](https://forum.qiime2.org/t/can-someone-help-me-to-decide-in-denoising-this-paired-end-sequence/29054 "2024-01-26T14:41:36Z")
**Posts on this page:** 7
**Page:** 1
### Author:  [@Mathi](https://forum.qiime2.org/u/Mathi)
#### Post date: [January 26, 2024, 2:41pm UTC](https://forum.qiime2.org/t/can-someone-help-me-to-decide-in-denoising-this-paired-end-sequence/29054/1 "2024-01-26T14:41:36Z")
Hello
i am a beginer in using qiime2 and i have some doubt in it. can you suggest me on what basis i have to denoise this paired end
[demux-paired.qzv](https://forum.qiime2.org/uploads/short-url/x03ZTDd7vWqvbt8I4lIHj4P56e8.qzv) (316.0 KB)
also my sole purpose of doing this is to perform a metagenome prediction in picrust. so do i have to rarefy or build relative abundance before implementing this feature table into picrust.(Note: i am using picrust in a new environment)
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### Author:  [@colinbrislawn](https://forum.qiime2.org/u/colinbrislawn)
#### Post date: [January 26, 2024, 2:54pm UTC](https://forum.qiime2.org/t/can-someone-help-me-to-decide-in-denoising-this-paired-end-sequence/29054/2 "2024-01-26T14:54:58Z")
Hello Mathi,
Welcome to the forums! :qiime2:
Thanks for posting the demux-paired.qzv file. The quality looks good and you are off to a great start!
I opened the .qzv file with httpw://view.qiime2.org and copied the image here:
How long is the amplicon you sequenced?
Have you tried running DADA2 already and what settings did you use?
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### Author:  [@Mathi](https://forum.qiime2.org/u/Mathi)
#### Post date: [January 26, 2024, 3:28pm UTC](https://forum.qiime2.org/t/can-someone-help-me-to-decide-in-denoising-this-paired-end-sequence/29054/3 "2024-01-26T15:28:51Z")
Thanks for your reply
is this is what you are asking and i had tried running dada2 with turncat len f = 264 and turncat len r = 192. i dont whether this is correct or wrong
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### Author:  [@Mathi](https://forum.qiime2.org/u/Mathi)
#### Post date: [January 26, 2024, 3:42pm UTC](https://forum.qiime2.org/t/can-someone-help-me-to-decide-in-denoising-this-paired-end-sequence/29054/4 "2024-01-26T15:42:53Z")
and this this the denoising stats i got after running dada2
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### Author:  [@colinbrislawn](https://forum.qiime2.org/u/colinbrislawn)
#### Post date: [January 26, 2024, 4:22pm UTC](https://forum.qiime2.org/t/can-someone-help-me-to-decide-in-denoising-this-paired-end-sequence/29054/5 "2024-01-26T16:22:50Z")
We have the data we need!
Use [this post](https://forum.qiime2.org/t/merging-quality-control-and-overlapping/12618/2) to estimate how long your amplicon will be, and how much overlap you will get with f=264 and r=192.
(This should explain why the 'percent of input merged' is \<20%.)
Looks like you have two samples. Do you have more samples or are you just starting with two?
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### Author:  [@Mathi](https://forum.qiime2.org/u/Mathi)
#### Post date: [January 26, 2024, 9:03pm UTC](https://forum.qiime2.org/t/can-someone-help-me-to-decide-in-denoising-this-paired-end-sequence/29054/6 "2024-01-26T21:03:39Z")
thankyou for your help and yes for now i am starting with just two
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### Author:  [@system](https://forum.qiime2.org/u/system)
#### Post date: [February 27, 2024, 3:04am UTC](https://forum.qiime2.org/t/can-someone-help-me-to-decide-in-denoising-this-paired-end-sequence/29054/7 "2024-02-27T03:04:26Z")
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