Im using qiime2-2020.8 on conda.
Im trying to perform ANCOM analysis to compare two samples, from high and low salinity. When I try to do so, I get the following error.
qiime composition ancom
Plugin error from composition:
All values in
groupingare unique. This method cannot operate on a grouping vector with only unique values (e.g., there are no ‘within’ variance because each group of samples contains only a single sample).
From this I understand that perhaps you can’t peform this analysis with only two samples.
Is my assumption correct?