I have started analysing my 16S data using qiime2. However regarding the sample metadata file I have a question. It might have been answered somewhere in the forum but I could not find. Sorry for duplicates.
I will get some important environmental variables’ results related to my samples later. So if I keep SampleID, BarcodeSequence, LinkerPrimerSequence and Description part same can I use different metadata file (for instance after including temperature or POC data to the table) for my beta diversity analysis.
To be more clear, I use metadata file 1 for qiime feature-table summarize and then add some environmental variables to matadata file 1 and use the second one for qiime diversity core-metrics-phylogenetic analysis. Will that create problem or not?
Since data I am dealing is big, I do not wanna analyse it over and over again.
Thanks in advance,