Can anyone solve q2-picrust2 plugin in the Qiime2-2018.11 version?

Community Plugin Support
1

Dear Sir,

I want to install the q2-picrust2 plugin in the Linux operating system having Qiime2-2018.11 version. However, I got errors after step 2. I have read similar topics in the qiime forum and followed the suggestions provided. However got the error. For the installation of q2-picrust2 plugin, I followed the website https://library.qiime2.org/plugins/q2-picrust2/13/ and completed the Step 1: ```
source activate qiime2-2018.11 and then step 2: conda install -c anaconda -c defaults -c conda-forge -c bioconda -c https://conda.anaconda.org/biocore q2-fragment-insertion
qiime dev refresh-cache
I didn’t get any error for step 1 & Step 2. However, when I used the commands from setup 3,

wget https://github.com/picrust/picrust2/releases/download/v2.0.3-b/picrust2-2.0.3-b.zip
unzip picrust2-2.0.3-b.zip
cd picrust2-2.0.3-b
conda-env update -n qiime2-2018.11 -f picrust2-env.yaml
pip install --editable .

I got an error and the error is as follows;

9.188.59, 13.250.177.223
Connecting to github.com|52.74.223.119|:443… connected.
HTTP request sent, awaiting response… 302 Found
Location: https://github-production-release-asset-2e65be.s3.amazonaws.com/123145877/1d335780-d22f-11e8-9960-d49c1e75251b?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIAIWNJYAX4CSVEH53A%2F20181205%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Date=20181205T014353Z&X-Amz-Expires=300&X-Amz-Signature=fded7a82b02b1f8118e9fcb3ad9683e69279a210bc891ccb84d155932352f800&X-Amz-SignedHeaders=host&actor_id=0&response-content-disposition=attachment%3B%20filename%3Dpicrust2-2.0.3-b.zip&response-content-type=application%2Foctet-stream [following]
–2018-12-05 09:43:43-- https://github-production-release-asset-2e65be.s3.amazonaws.com/123145877/1d335780-d22f-11e8-9960-d49c1e75251b?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIAIWNJYAX4CSVEH53A%2F20181205%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Date=20181205T014353Z&X-Amz-Expires=300&X-Amz-Signature=fded7a82b02b1f8118e9fcb3ad9683e69279a210bc891ccb84d155932352f800&X-Amz-SignedHeaders=host&actor_id=0&response-content-disposition=attachment%3B%20filename%3Dpicrust2-2.0.3-b.zip&response-content-type=application%2Foctet-stream
Resolving github-production-release-asset-2e65be.s3.amazonaws.com… 52.216.107.212
Connecting to github-production-release-asset-2e65be.s3.amazonaws.com|52.216.107.212|:443… connected.
HTTP request sent, awaiting response… 200 OK
Length: 134723266 (128M) [application/octet-stream]
picrust2-2.0.3-b.zip: Permission denied

Cannot write to ‘picrust2-2.0.3-b.zip’ (Success).
unzip picrust2-2.0.3-b.zip
unzip: cannot find or open picrust2-2.0.3-b.zip, picrust2-2.0.3-b.zip.zip or picrust2-2.0.3-b.zip.ZIP.

Waiting for a positive response

Thanks

2

I guess you do not have root privileges, for writing the file...

2 Likes
3

Dear Sir,

Any suggestions, I am running the setup from a server having Linux operating system.

Thanks

4

Download the picrust2-2.0.3-b.zip file via a browser…
Copy the file to /home directory (if you have access), else put it in any directory where you have access (Change the terminal to this directory)…
Skip the wget step, as you have already downloaded the file…
Start with unzip command…
This should work fine, I guess.

You may also face the same problem while installing the Plugin at “Step 5”…
Try to do the same thing…

Or
Get the root password from Admin…

2 Likes
5

Dear Sir,

Thank You. I will try and let you know!.

Thanks

6

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