Building a COI database from BOLD references

[SoilRotifer (Mike Robeson)]

Hi @PanZhu921,

@PanZhu921

Can I use the "bold_anml_classifier. qza" classifier provided by you for taxonomy annotation.Is that work?

I would not suggest doing this, as the primer sequences can affect how these amplicon regions are extracted from the sequence reference database. Potentially affecting your ability to classify your reads appropriately.

I would use your own primers to extract the amplicon region. You can also supplement parts of the BOLD tutorial, with this tutorial.