# BOM in Manifest **URL:** \ **Category:** User Support\ **Tags:** import, fastq-manifest\ **Created:** [September 17, 2018, 1:43pm UTC](https://forum.qiime2.org/t/bom-in-manifest/6061 "2018-09-17T13:43:40Z")\ **Posts on this page:** 6\ **Page:** 1
**Author:** ![Cybele\_C](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/c/65b543/32.png) [@Cybele\_C](https://forum.qiime2.org/u/Cybele_C)\ **Post date:** [September 17, 2018, 1:43pm UTC](https://forum.qiime2.org/t/bom-in-manifest/6061/1 "2018-09-17T13:43:40Z")
Hello! There does seem to be a problem with my manifest file. I tried the absolute file path, though it did seem to be in the correct directory (at bottom). I altered the names of the raw files so that they don't have the S## designation, but otherwise I can't see a problem: [ManifestRun2.csv](https://cdck-file-uploads-global.s3.dualstack.us-west-2.amazonaws.com/flex002/uploads/qiime21/original/2X/0/0e3e4d1409c8522b052c208cd54b0876de245d20.csv) (1.2 KB) qiime tools import \ \> --type 'SampleData[PairedEndSequencesWithQuality]' \ \> --input-path ManifestRun2.csv \ \> --output-path paired-end-demux2.qza \ \> --source-format PairedEndFastqManifestPhred33 **There was a problem importing ManifestRun2.csv:** **ManifestRun2.csv is not a(n) PairedEndFastqManifestPhred33 file** (qiime2-2018.6) Cybeles-MacBook-Pro:qiime2-skate3 cybelecollins$ qiime tools import \ \> --type 'SampleData[PairedEndSequencesWithQuality]' \ \> --input-path /Users/cybelecollins/qiime2-skate3/ManifestRun2.csv \ \> --output-path paired-end-demux2.qza \ \> --source-format PairedEndFastqManifestPhred33 **There was a problem importing /Users/cybelecollins/qiime2-skate3/ManifestRun2.csv:** **/Users/cybelecollins/qiime2-skate3/ManifestRun2.csv is not a(n) PairedEndFastqManifestPhred33 file** ---
**Author:** ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)\ **Post date:** [September 17, 2018, 6:14pm UTC](https://forum.qiime2.org/t/bom-in-manifest/6061/2 "2018-09-17T18:14:48Z")
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**Author:** ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)\ **Post date:** [September 17, 2018, 6:17pm UTC](https://forum.qiime2.org/t/bom-in-manifest/6061/3 "2018-09-17T18:17:27Z")
Hey there @Cybele_C! > [@Cybele\_C](#): > > /Users/cybelecollins/qiime2-skate3/ManifestRun2.csv is not a(n) PairedEndFastqManifestPhred33 file Okay, I took a look at the attached file, and to quote @ebolyen: > [@Error : not a phred 33 file](https://forum.qiime2.org/t/error-not-a-phred-33-file/3356/7): > > Your file contains what is called a [Byte Order Mark](https://en.wikipedia.org/wiki/Byte_order_mark) at the very beginning (it’s completely invisible and meant only for the computer to read). We typically expect files to be encoded as UTF-8 (you may have heard of ASCII, this is the same thing, just more universal). While UTF-8 is ok with a byte order mark it isn’t actually useful since there is no byte order in UTF-8 (vs something like UTF-16). > > QIIME 2 shouldn’t have given you any trouble for having the BOM, so there’s definitely something wrong on our end. I’ll try and figure out where an issue will go. > > In the meanwhile, here’s a file with the BOM stripped: [ManifestRun2.csv](https://cdck-file-uploads-global.s3.dualstack.us-west-2.amazonaws.com/flex002/uploads/qiime21/original/2X/c/cb6c636f5266d515e1b495f574cbd5684cda9179.csv) (1.2 KB) I removed the BOM, and attached above. Give that a shot and let us know! @ebolyen and I are planning on addressing this during the 2018.10 development cycle, so stay tuned! :qiime2: 🦖 ---
**Author:** ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)\ **Post date:** [September 17, 2018, 6:17pm UTC](https://forum.qiime2.org/t/bom-in-manifest/6061/4 "2018-09-17T18:17:29Z")
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**Author:** ![Cybele\_C](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/c/65b543/32.png) [@Cybele\_C](https://forum.qiime2.org/u/Cybele_C)\ **Post date:** [September 18, 2018, 2:04am UTC](https://forum.qiime2.org/t/bom-in-manifest/6061/5 "2018-09-18T02:04:21Z")
Hello! I tried my original manifest file in text edit after pressing the delete button at the beginning. This worked. Programming is very mysterious, but becoming more intriguing - deep into Wikipedia right now. Thank you for the new information and the help. I appreciate it so much. ---
**Author:** ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)\ **Post date:** [October 19, 2018, 8:04am UTC](https://forum.qiime2.org/t/bom-in-manifest/6061/6 "2018-10-19T08:04:24Z")
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