bray_curtis_distance_matrix.qza (76.1 KB)
sample-metadata.tsv (9.2 KB)
Hi i'm going through the Atacama tutorial and trying to run the Bioenv command to and answer the question
What sample metadata or combinations of sample metadata are mostly strongly associated with the differences in microbial composition of samples?
i'm running into the following error:
qiime diversity bioenv \
--i-distance-matrix core-metrics-results/bray_curtis_distance_matrix.qza
--m-metadata-file sample-metadata.tsv
--o-visualization bray_curtis_atacama.qzv
Plugin error from diversity:
Column(s) in the data frame could not be scaled, likely because the column(s) had no variance.
I've looked through the metadata file and there is more than 1 different value in each column - so it seems like variance isn't the issue?
However there are some cells which are missing - no values - could this be the problem?
I've attached the sample metadata file i'm working with and the core-metric file
Would appreciate any suggestions - Thanks