Bioenv Error - Atacama Tutorial

bray_curtis_distance_matrix.qza (76.1 KB)

sample-metadata.tsv (9.2 KB)

Hi i'm going through the Atacama tutorial and trying to run the Bioenv command to and answer the question

What sample metadata or combinations of sample metadata are mostly strongly associated with the differences in microbial composition of samples?

i'm running into the following error:

qiime diversity bioenv \

--i-distance-matrix core-metrics-results/bray_curtis_distance_matrix.qza
--m-metadata-file sample-metadata.tsv
--o-visualization bray_curtis_atacama.qzv

Plugin error from diversity:

Column(s) in the data frame could not be scaled, likely because the column(s) had no variance.

I've looked through the metadata file and there is more than 1 different value in each column - so it seems like variance isn't the issue?
However there are some cells which are missing - no values - could this be the problem?

I've attached the sample metadata file i'm working with and the core-metric file

Would appreciate any suggestions - Thanks

Hi @Tohseef!
This is a known issue that is on our radar to fix. In the meantime, here’s a hacky solution that should keep you moving forward.

Chris :pig2:

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