Thank you for the suggestion. As per your suggestion I used “state” variable as the extraction method where state - 1 is method 1 and state-2 is method2 and my group column as the primer. I gave the studyid the name of the samples, that is both my SampleID and StudtyID are same. Following was the command I used:
qiime longitudinal pairwise-distances --i-distance-core-metrics-results/weighted_unifrac_distance_matrix.qza --m-metadata-file.tsv --p-group-column 16S_Regions --p-state-column ExtractionMethod --p-state-1 Swab_DNA --p-state-2 Soil_DNA --p-individual-id-column StudyID --p-replicate-handling random --o-visualization core-metrics-results/pairwise-distance-primer-extractionmethod.qzv
After running this command when opening the qzv file I am not getting a boxplot figure. The resulting figure is blank with my variables labelled. Should I post this in the technical section as a new post? Could you please help me to resolve this problem.
Is there anyway that I could export the unifrac weighted distance matrix to a csv. If this option is there then it will be very helpful.
I have attached the unifrac_weighted_distancematrix, qzv file and metadata here
weighted_unifrac_distance_matrix.qza (151.5 KB)
metadata.tsv (1.3 KB)
pairwise-distance-extraction_method-16S_Regions.qzv (352.3 KB)