# Best analysis to do after CCA?
**URL:** https://forum.qiime2.org/t/best-analysis-to-do-after-cca/32889
**Category:** General Discussion
**Created:** [April 3, 2025, 11:52am UTC](https://forum.qiime2.org/t/best-analysis-to-do-after-cca/32889 "2025-04-03T11:52:36Z")
**Posts on this page:** 2
**Page:** 1
### Author:  [@PabloCL](https://forum.qiime2.org/u/PabloCL)
#### Post date: [April 3, 2025, 11:52am UTC](https://forum.qiime2.org/t/best-analysis-to-do-after-cca/32889/1 "2025-04-03T11:52:36Z")
Hello everyone,
What is the best statistical analysis to continue after a Canonical Correspondence Analysis? I have carried a CCA , and found significant effect of Temperature (a continuous, numerical variable) on the bacterial community found in these samples. Now that I know that temperature has a significant effect on the bacterial community, I would like to analyze it in isolation and see which ASVs exactly are significantly affected, and how, by temperature, but I can't seem to find a proper statistical test that will let me test the effect of one numerical independent variable on several dependent proportion variables. Maybe there is something I can do with the CCA scores?
Thanks a lot in advance for your help!
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### Author:  [@jphagen](https://forum.qiime2.org/u/jphagen)
#### Post date: [April 14, 2025, 6:25pm UTC](https://forum.qiime2.org/t/best-analysis-to-do-after-cca/32889/2 "2025-04-14T18:25:22Z")
Hi @PabloCL,
I am not personally familiar with CCA scores, however, I do not believe there is a way to use them in QIIME2 directly at this time. We will take your interest in this into account for future development!
We do have a [workflow for longitudinal data](https://docs.qiime2.org/2024.10/tutorials/longitudinal/) that you may be interested in! check out the [linear-mixed-effect-models](https://docs.qiime2.org/2024.10/tutorials/longitudinal/#linear-mixed-effect-models)
--Hannah