Atacama Soil Tutorial Obstacle

(adinsmoor) #1

Hello–I have worked through the moving picture tutorial, but I am getting stuck with obtaining the data for the Atacama Soil Tutorial, shown:

(base) wirelessprv-10-193-20-174:qiime2-atacama-tutorial adinsmoor$ wget \

-O “emp-paired-end-sequences/forward.fastq.gz”
https://data.qiime2.org/2019.4/tutorials/atacama-soils/10p/forward.fastq.gz
emp-paired-end-sequences/forward.fastq.gz: No such file or directory

I included my conda info. below:

(base) wirelessprv-10-193-20-174:qiime2-atacama-tutorial adinsmoor$ conda info

 active environment : base
active env location : /Users/adinsmoor/miniconda2
        shell level : 2
   user config file : /Users/adinsmoor/.condarc

populated config files :
conda version : 4.6.14
conda-build version : not installed
python version : 2.7.16.final.0
base environment : /Users/adinsmoor/miniconda2 (writable)
channel URLs : https://repo.anaconda.com/pkgs/main/osx-64
https://repo.anaconda.com/pkgs/main/noarch
https://repo.anaconda.com/pkgs/free/osx-64
https://repo.anaconda.com/pkgs/free/noarch
https://repo.anaconda.com/pkgs/r/osx-64
https://repo.anaconda.com/pkgs/r/noarch
package cache : /Users/adinsmoor/miniconda2/pkgs
/Users/adinsmoor/.conda/pkgs
envs directories : /Users/adinsmoor/miniconda2/envs
/Users/adinsmoor/.conda/envs
platform : osx-64
user-agent : conda/4.6.14 requests/2.21.0 CPython/2.7.16 Darwin/18.5.0 OSX/10.14.4
UID:GID : 501:20
netrc file : None
offline mode : False

(base) wirelessprv-10-193-20-174:qiime2-atacama-tutorial adinsmoor$

Thanks!

(Matthew Ryan Dillon) #2

Hey there @adinsmoor!

Let’s take a closer look at the error message:

This suggests that the emp-paired-end-sequences directory either didn’t get made, or, it was made in a different location. Please carefully double-check that you are running all of the commands specified in the tutorial, and, in the same order that the tutorial provides them. So, my suggestion is to go back to the beginning of the tutorial and make sure you follow the instructions, you should wind up with the directory you need, in the location you need it.

Thanks! :qiime2:

(adinsmoor) #3

I restarted the window and realized that I also had to create a folder named “emp-paired-end-sequences” within the tutorial folder in my finder to locate and bypass the step I was stuck on. Everything is working well now. Thank you!

Andrew

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