Atacama Soil Microbiome Tutorial - problems creating the import artfact

[malenaamer (Malena)]

Hello,
I’m following the Atacama Soil Tutorial, I’ve downloaded the 3 fastq.gz files and named them as indicated. When I try to create the artifact it shows me the following error:

(qiime2-2017.2) MacBook-Pro-de-Malena-2:paired_seq_tutorial mae$ qiime tools import    --type EMPPairedEndSequences    --input-path emp-paired-end-sequences    --output-path emp-paired-end-sequences.qza
Usage: qiime tools import [OPTIONS]

Error: Invalid value for "--input-path": Path "emp-paired-end-sequences" does not exist.

Thanks for your help!

[ebolyen (Evan Bolyen)]

Hi @malenaamer,

I suspect the 3 fastq.gz files you mentioned are either not in a directory containing only those files, or are in a directory with a different name than emp-paired-sequences. The name doesn’t really matter, but that is what the error you are getting is trying to say, it can’t find a directory as described by --input-path which has that name.

[malenaamer (Malena)]

That was the problem, I had named the files as:

emp-paired-end-sequences/filename.fastq.gz instead of creating the folder.

Thanks Evan!