# ASV's ID's question

**URL:** https://forum.qiime2.org/t/asvs-ids-question/21816
**Category:** General Discussion
**Tags:** taxonomy, dada2
**Created:** [January 7, 2022, 11:35pm UTC](https://forum.qiime2.org/t/asvs-ids-question/21816 "2022-01-07T23:35:44Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![Steph\_Hp](https://forum.qiime2.org/user_avatar/forum.qiime2.org/steph_hp/32/4147_2.png) [@Steph\_Hp](https://forum.qiime2.org/u/Steph_Hp)
#### Post date: [January 7, 2022, 11:35pm UTC](https://forum.qiime2.org/t/asvs-ids-question/21816/1 "2022-01-07T23:35:44Z")

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Hello!  
I have a general question about ASV's.  
I read the article and it was not clear to me how ASV's ID's are assigned! I mean, is this random ?  
My question comes from the idea that I have my data in different libraries and I run DADA2 for each one. So, at the end I merged the tables and sequences that came from each run/library. So, If the ASV's ID's are different assigned for each taxon I don't know if this issue could affect beta diversity calculations.  
If so, do I have to clustered my ASV's into OTU's with the cluster de novo approach? or maybe to cluster into a level like genus ? The effect of batch in this case may be a problem for me with the diversity calculations?

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### Author: ![timanix](https://forum.qiime2.org/user_avatar/forum.qiime2.org/timanix/32/17879_2.png) [@timanix](https://forum.qiime2.org/u/timanix)
#### Post date: [January 8, 2022, 11:59am UTC](https://forum.qiime2.org/t/asvs-ids-question/21816/2 "2022-01-08T11:59:34Z")

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Hello!  
I believe [this topic](https://forum.qiime2.org/t/how-are-asv-ids-generated/6075) is highly relevant to your questions, so you can read excellent explanation provided there. Please, feel free to ask here additional questions if they are not covered by the link.

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### Author: ![Steph\_Hp](https://forum.qiime2.org/user_avatar/forum.qiime2.org/steph_hp/32/4147_2.png) [@Steph\_Hp](https://forum.qiime2.org/u/Steph_Hp)
#### Post date: [January 11, 2022, 10:50pm UTC](https://forum.qiime2.org/t/asvs-ids-question/21816/3 "2022-01-11T22:50:19Z")

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Thanks for your answer!
