# ASCII error while impoting manifest

**URL:** https://forum.qiime2.org/t/ascii-error-while-impoting-manifest/12486
**Category:** Technical Support
**Created:** [November 13, 2019, 10:24pm UTC](https://forum.qiime2.org/t/ascii-error-while-impoting-manifest/12486 "2019-11-13T22:24:33Z")
**Posts on this page:** 6
**Page:** 1

<div class="post-metadata">

### Author: ![HebaHussein-1981](https://forum.qiime2.org/user_avatar/forum.qiime2.org/hebahussein-1981/32/6118_2.png) [@HebaHussein-1981](https://forum.qiime2.org/u/HebaHussein-1981)
#### Post date: [November 13, 2019, 10:24pm UTC](https://forum.qiime2.org/t/ascii-error-while-impoting-manifest/12486/1 "2019-11-13T22:24:33Z")

</div>

I keep on getting this ASCII related error. I tried changing the dashes into small but still getting it. I am importing manifest file and using qiime2-2019.7 I searched the forums and tried many things but still  
thanks qiime tools import --type ‘SampleData[SingleEndSequenceWithQuality]’ --input-path/Users/hebahussein/Desktop/Data --output-path/Users/hebahussein/Desktop/single-end-demux.qza --input-formatSingleEndFastqManifestPhred33V2

**Error: Detected invalid character in: ‘SampleData[SingleEndSequenceWithQuality]’**

**Verify the correct quotes or dashes (ASCII) are being used.**

---

<div class="post-metadata">

### Author: ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [November 13, 2019, 10:38pm UTC](https://forum.qiime2.org/t/ascii-error-while-impoting-manifest/12486/2 "2019-11-13T22:38:52Z")

</div>

Hi @HebaHussein-1981, the issue are these two characters:

> [@HebaHussein-1981](#):
>
> ‘

> [@HebaHussein-1981](#):
>
> ’

Replace both with the `'` character! :qiime2:

---

<div class="post-metadata">

### Author: ![HebaHussein-1981](https://forum.qiime2.org/user_avatar/forum.qiime2.org/hebahussein-1981/32/6118_2.png) [@HebaHussein-1981](https://forum.qiime2.org/u/HebaHussein-1981)
#### Post date: [November 13, 2019, 11:23pm UTC](https://forum.qiime2.org/t/ascii-error-while-impoting-manifest/12486/3 "2019-11-13T23:23:29Z")

</div>

> [@thermokarst](#):
>
> Replace both with the `'` character!

thanks I dont know why i got more errors. I think because I need to copy and paste only the forward FASTQ files because each forward and reverse in the same folder.  
I am doing singleend  
**(1/3?) no such option: --input-path/Users/hebahussein/Desktop/Data**

**(2/3?) no such option: --output-path/Users/hebahussein/Desktop/single-end-**

**demux.qza**

**(3/3?) no such option: --input-formatSingleEndFastqManifestPhred33V2**

---

<div class="post-metadata">

### Author: ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [November 18, 2019, 2:27pm UTC](https://forum.qiime2.org/t/ascii-error-while-impoting-manifest/12486/4 "2019-11-18T14:27:45Z")

</div>



---

<div class="post-metadata">

### Author: ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [November 18, 2019, 11:53pm UTC](https://forum.qiime2.org/t/ascii-error-while-impoting-manifest/12486/5 "2019-11-18T23:53:35Z")

</div>

Hi @HebaHussein-1981!

> [@HebaHussein-1981](#):
>
> I think because I need to copy and paste only the forward FASTQ files because each forward and reverse in the same folder.

No, that isn't the case. The errors presented here are because the commands are invalid, they contain typos in them. This has been an [issue before](https://forum.qiime2.org/t/importing-paired-fastq-file-into-qiime-had-errors-any-help/12113/) for you - I suggest you take a few minutes to review the command you have written, and compare that to what the error message tell you --- all the answers are there for you! If you get stuck, well, you know where to find us!

:qiime2:

---

<div class="post-metadata">

### Author: ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [November 18, 2019, 11:53pm UTC](https://forum.qiime2.org/t/ascii-error-while-impoting-manifest/12486/6 "2019-11-18T23:53:38Z")

</div>


