Dear Sergio Alías-Segura,
First of all, I would like to express my gratitude.
While independently studying microbiome analysis with Qiime2 by following the tutorial, I found myself unfamiliar with terminal commands.
I also realized, after much research, that the .condarc file you mentioned is not visible on my system. (Configuring Conda – Introduction to Conda for (Data) Scientists)
I tried changing the channel priority order, updating the conda version, and attempting various other methods, including using bcftools. During these attempts, I noticed that the error related to ANCOMBC occasionally changed. I spent a considerable amount of time searching for solutions to the dyld: Library not loaded issue.
In the end, I decided on one solution: I deleted everything. I uninstalled miniconda3, R, R-studio, and everything else.
Afterward, I reinstalled miniconda and Qiime2 2024.5, then installed R anew. I followed the ANCOM-BC method from the moving-pictures-tutorial, and this time, it worked.
For users experiencing similar issues, I recommend uninstalling miniconda3 completely using the method provided here (https://youtu.be/dKGIjTHyVAg?si=MDNrUeIP4LxW0puB) and then reinstalling it. Additionally, uninstall and reinstall R completely.
Thank you, Sergio Alías-Segura, for taking the time to help resolve my issue despite your busy schedule.
Sincerely,
Byunghun So