May I add another question to this ANCOM thread. We have analysed 16S amplicons in laboratory scale bioreactors for ca. 2 month; samples were taken at day, 17, 32, and 50, (day after satellite start; DASS) respectively, from 5 independent reactors.
We observed that the microbial community started to segregate over time, and the communities at day 50 were considerably different.
In order to identify the most differing taxa at the various time points, we first filtered the samples by the time points and did independent ancom analysis only for the samples at the various time points.
W considerably increased from day 17 to 50, as well as clr increased, because the differences in the community were greated at day 50, which was already visible in beta diversity analyses.
However, at day 50, only one single taxon was listed in the ancom statistical results section next to the ANCOM volcano plot, while more taxa were flagged at day 32 and 17 as true (reject null hypothesis). This does not make sense to me. If W and clr increase both, then I would expect more taxa listed in the ancom statistics table, and not less.
I have added the three visualizations below. ancom_Ferm-Type_L7_DASS-17_addpc.qzv (449.8 KB) ancom_Ferm-Type_L7_DASS-32_addpc.qzv (429.7 KB) ancom_Ferm-Type_L7_DASS-50_addpc.qzv (428.4 KB)