ANCOM rejects null hypothesis at many W values that are 0

Hi @fgara,

Glad it helped!

Some of this is anecdotal. I suspect that if you go back to that earlier analysis and look, you'll find a set of specific features that drive that phylum level difference. (It's also worth keeping in mind that taxonomy is nested.). I dont think its a great example of a paper or how to do analysis, but if you look at Table 3 of this study, and you look up the taxonomy associated with each level, you'll find that it's nested and the difference in Fusobacteria is driven by the difference in Leptotrichia. (However, I dont recommend using the paper as an example for much else, I dont think they did a great job with respect to that nesting aspect.)

I think there's also an ecological question - is it important to know that global warming is associated with a change in the number of things with spinal cords :woman_scientist::shark::dog2::bird::frog: and the number of things with shells :lobster::spider::bug:, or is it more helpful to know a specific set of species within phylum chordata is driving CO2 and CH4 production :woman_scientist: ::cow2:? I think community level data is really important, don't get me wrong, but Im not sure that collapsing to that high of level works as well as alternative statistics (alpha & beta diversity).

Best,
Justine

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