sorry I want to run ANCOM for my data which contains features from 3 different kinds of meat. as you mentioned in the moving pictures tutorial I filtered the data to only beef (for example).
the metadata includes just 3 columns: sample-id, meat-kind and sample.
after I filtered and used psudocount command, when I wanted to run ANCOM there is an error which is: All values in
grouping are unique
I cannot understand what’s the problem because I think its just like the moving pictures tutorial. you filtered to the body site and just analyzed gut samples as same as I did I just tried to analyze beef samples. you chose the “subject” column for ANCOM which includes subject1 and subject2 and I chose the “sample” column which includes beef1, beef2, beef3, and beef4.
could you please let me know what’s the difference and what’s the problem and how can I run ANCOM for my data?
qiime feature-table filter-samples
qiime composition add-pseudocount
qiime composition ancom
but the error is:
All values in
grouping are unique. This method cannot operate on a grouping vector with only unique values (e.g., there are no ‘within’ variance because each group of samples contains only a single sample).
Debug info has been saved to /tmp/qiime2-q2cli-err-1t86ltl_.log
thank you in advance.