ANCOM Empty Metadata Column Error

[ninaxhua (Nina Hua)]

Hello,

I’m trying to apply differential abundance testing with ANCOM. However, I ran into an error when I ran the following:
qiime composition ancom \ --i-table $Q2P/comp-material-table.qza \ --m-metadata-file $Q2P/CC-trial1-metadata.tsv \ --m-metadata-column Material \ --o-visualization $Q2P/ancom-CC-Material.qzv

Error:
Plugin error from composition:

All IDs were filtered out of the MetadataColumn, resulting in an empty MetadataColumn object.

Debug info has been saved to /var/folders/zs/30xfcww916qb84m7xvd0pv240000gn/T/qiime2-q2cli-err-xmxcqkta.log

I’m not sure of how to interpret this error?

Thanks!
Nina

[ebolyen (Evan Bolyen)]

Hi @ninaxhua,

The error is telling you (in a roundabout way) that the IDs don’t match your table. More specifically the IDs of the column (from CC-trial1-metadata.tsv) are being restricted to the IDs in the table, but there weren’t any left after doing that, meaning none of them match.

Hope that helps!