I am currently trying the QIAseq 16S/ITS Panels in the lab, on a Illumina MiSeq platform and was wondering if I can upload files from those runs to Qiime2 for analysis and interpretation…is it the right place to ask this question?
Thank you in advance for your time and help!
Totally not a silly question! If Qiagen has a reconstruction algorithm of some kind that generates a feature table and taxonomic assignments, you can import those. Maybe you’ll get a phylogenetic tree . If you have taxonomy and a feature table, you can run all kinds of non-phylogenetic analyses once you get the data loaded.
Currently, the only open source reconstruction algorithm I know of is SMURF, a Matlab-based implementation which is pretty hard to use. There isn’t any QIIME plugin AFAIK that does reconstruction (but you can always check the library!)
Thank you very much Justine…I will try and let you know!
All the best
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