# Analysis workflow **URL:** https://forum.qiime2.org/t/analysis-workflow/15307 **Category:** General Discussion **Tags:** taxonomy **Created:** [June 9, 2020, 2:07pm UTC](https://forum.qiime2.org/t/analysis-workflow/15307 "2020-06-09T14:07:11Z") **Posts on this page:** 3 **Page:** 1
### Author: ![Anas\_S](https://forum.qiime2.org/user_avatar/forum.qiime2.org/anas_s/32/8305_2.png) [@Anas\_S](https://forum.qiime2.org/u/Anas_S) #### Post date: [June 9, 2020, 2:07pm UTC](https://forum.qiime2.org/t/analysis-workflow/15307/1 "2020-06-09T14:07:11Z")
Hi everyone ! My name is Anas, i am currently a trainee and i have been given 16s sequences (fastq.gz) to analyze (they came from healthy vs diseased mussels). Qiime2 seemed to be the best way for me to perform this analysis, so i went through the "moving pictures" and the atacama soil microbiome tutorials with success. I have very basic knowledge about microbiome analysis but no experience in this topic. Therefore **i am asking for help in order to guide me through the workflow of the analysis**. From my sequences (paired end demultiplexed), i would like to generate figures as follows: ![image](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/2X/8/803b20da67325f22acf69e4437231f6047322014.png) and ![image](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/2X/6/6fe52fa30c7248a91086e99116d2c409861026c7.png) Here is my metadata file, validated with keemei ![image](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/2X/0/0370a2d1eda7d6aaabe2192923bd818a2f9a746d.png) My purpose is to compare, diversity between healthy and diseased mussels and to point out taxas that may cause the disease. Thank you in advance for your help ! -- Anas ---
### Author: ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst) #### Post date: [June 9, 2020, 2:11pm UTC](https://forum.qiime2.org/t/analysis-workflow/15307/2 "2020-06-09T14:11:18Z")
A post was merged into an existing topic: [Analysis workflow](https://forum.qiime2.org/t/analysis-workflow/15304) ---
### Author: ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst) #### Post date: [June 9, 2020, 2:11pm UTC](https://forum.qiime2.org/t/analysis-workflow/15307/3 "2020-06-09T14:11:18Z")