# Am I interpreting weighted vs. unweighted distance matrix unifrac boxplots correctly?

**URL:** https://forum.qiime2.org/t/am-i-interpreting-weighted-vs-unweighted-distance-matrix-unifrac-boxplots-correctly/27082
**Category:** User Support
**Created:** [July 16, 2023, 2:16am UTC](https://forum.qiime2.org/t/am-i-interpreting-weighted-vs-unweighted-distance-matrix-unifrac-boxplots-correctly/27082 "2023-07-16T02:16:02Z")
**Posts on this page:** 1
**Showing post:** 21

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### Author: ![cherman2](https://forum.qiime2.org/user_avatar/forum.qiime2.org/cherman2/32/17129_2.png) [@cherman2](https://forum.qiime2.org/u/cherman2)
#### Post date: [July 25, 2023, 3:02pm UTC](https://forum.qiime2.org/t/am-i-interpreting-weighted-vs-unweighted-distance-matrix-unifrac-boxplots-correctly/27082/21 "2023-07-25T15:02:19Z")

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Hi @macrobiome,

> [@macrobiome](#):
>
> definitely hit enter haha

Good! I always have to check 😅

> [@macrobiome](#):
>
> Is there a problem with how the metadata file columns are set up?

I misunderstood how your metadata was set up. There is no issue with your donor metadata column.  
However you will want to add a "fake" time column. I would put a column that is called week in your metadata. Give all the tumor values 1 and leave all the intestines blank. (This is part of the tweaking this method to fix your study)

Lastly remove the filtering parameter, you will need all your samples in order to run this anaysis.

```auto
--p-where '[SampleType]'="Tumor"' 

```

Can you try those suggestions and let me know how it goes. If this doesn't work for you would you share your faith\_pd\_vector with me so that I can try it? (You can DM me your data if your want)  
Hope that helps!  
🐢

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