Dear Sir,

I have read the sampling depth and alpha rarefaction plotting from the moving picture tutorials. Both have confused me. For diversity analysis, we apply `--p-sampling-depth`

, which randomly subsample the counts from each sample. We are looking at the sequence count in table.qzv. If sequence counts in certain samples is less than 1000, we do not pass them to diversity analysis. After diversity analysis in moving pictures tutorials, a small portion is about the rarefaction plotting, where --p-max-depth is put from the frequency per sample in the Table.qzv particularly near to median frequency. We got two plots alpha rarefaction plots and bottom plots. I am very much confused between the sampling depth and alpha rarefaction plottings. The question is where we have to put the sampling depth and alpha rarefaction plotting when doing analysis and what is the importance. Is there any difference between them? I have seen several rarefied curves in the literature. Why we rarefied after diversity analysis? What is Waiting for a positive response.

Thanks