# Alpha Group Significance Error

**URL:** https://forum.qiime2.org/t/alpha-group-significance-error/4468
**Category:** User Support
**Tags:** group-significance, alpha
**Created:** [June 6, 2018, 12:49pm UTC](https://forum.qiime2.org/t/alpha-group-significance-error/4468 "2018-06-06T12:49:07Z")
**Posts on this page:** 1
**Showing post:** 5

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### Author: ![bye\_ology](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/b/bc8723/32.png) [@bye\_ology](https://forum.qiime2.org/u/bye_ology)
#### Post date: [June 7, 2018, 2:59pm UTC](https://forum.qiime2.org/t/alpha-group-significance-error/4468/5 "2018-06-07T14:59:21Z")

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Hi Matthew! I've set my sample depth super low because I was testing different trim lengths with deblur and was using a very high number. I've also attached my metadata to this reply. Thank you so much!

[sample-metadata.tsv](https://cdck-file-uploads-global.s3.dualstack.us-west-2.amazonaws.com/flex002/uploads/qiime21/original/2X/0/026d7df2c64751e05ccec627053fb545fc21be68.tsv) (598 Bytes)

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