alpha diversity group significance (Kruskal-wallis):2+groups?

sorry, I’m not sure what you mean.
And I also want know. If the data is normal distribution,I should select ANOVA, if it is not, I should select KW,isnt it?

Now, I have 4 groups samples, and each has 3 subgroups. And I want compare 3 subgroups difference in a group, so run the
Test of Homogeneity of Variances in SPSS, I find a group isnt normal normal distribution but other 3 group is . So, do I choose KW for the sake of uniformity? or 3 groups use ANOVA ,and the rest one use KW?