I used the down commands to calculate the core-metrics-results especially I am interested in calculating evenness and Shannon index, I only have one sample with depth 10.000 and after i run the down command i had this error"/tmp/q2-AlphaDiversityFormat-rlwh7ghy is not a(n) AlphaDiversityFormat file" and i do not understand where is the problem
Do not run this command if you only have one sample, because the outputs will not make sense. Most diversity analyses (most importantly those used by this pipeline) are only useful and/or will only compute if you have > 1 sample.
I recommend instead you rarefy your feature table and use qiime diversity alpha to measure alpha diversity of your single sample.
DearNicholas_Bokulich thank you for your reply, I am new in using qiime could please explain more what you main by measure, there is another way to measure it or you mean use OTU table and do in R ?
table-no-mitochondria-no-chloroplast.qza (242.0 KB)
this is the table, as i told you i was trying to run the analysis to calculate both Shanon and pielou but unfortunately. thanks for your fast reply.
@Nizar_Goda,
Please install the latest version of QIIME 2 and try again. You appear to be suffering from a format validation bug in older releases of QIIME 2 that has been fixed. I have confirmed that your data work with the latest release.
Check out the tutorials for examples of how to use the alpha diversity data, e.g., see the moving pictures tutorial. Note, however, that not all visualizations may work since you only have one sample.
You may want to check out the tutorial on exporting data too. Since you have 1 samples, there is nothing to visualize, per se, and you may just want to look at the raw value.