Alpha and Beta diversity problem with metadata

Hello,
I have a problem with alpha and beta diversity. I am trying to run:

qiime diversity core-metrics-phylogenetic \
  --i-phylogeny rooted-tree.qza \
  --i-table table.qza \
  --p-sampling-depth 162 \
  --m-metadata-file metadata-chronic.tsv \
  --output-dir core-metrics-results

but I get the following:

_**Error: no suchoption: --m-metadata-file**_

My metadata file name is metadata-chronic.tvs
Do I have problems with my metadata? also here is my table.qzatable.qza (10.2 KB)
Thank you much in advance!!

-Alba Mayta

Hey @acmayta!

That’s really strange. Would you be able to provide a screenshot of your command and the error? What you’ve got should be working.

Just a quick follow-up @acmayta — the --m-metadata-file parameter was added in QIIME 2 2017.10 — if you are running a version of QIIME 2 that is older than 2017.10, that would explain this error. In addition to what @ebolyen requested, can you please confirm that you are using QIIME 2 2017.10? If you aren’t please check out the install guide on instructions for installing this version! :t_rex:

2 Likes

This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.