all fastq files in fastq.gz vs manifest file

I believe I have multiple forward samples in one forward fastq file and one reverse fastq file, verses qiimee2 tutoral which shows one fastq file per sample. How do I create a manifest file when all fastq files/ samples are in one forward file and reverse file (fastq.gz).
link to the data https://figshare.com/s/721763cb9a0afd00d049
I appreciate your help!

Hi. I believe you need to take a look at this user documentation to create manifest file for your run files:

https://docs.qiime2.org/2020.6/tutorials/importing/

You should follow the “Fastq manifest” formats" section to import your data into qiime2.

1 Like

@qamrq25 is pointing you in the right direction, @AYK. Take some time with that tutorial, and feel free to reply here if you run into further problems importing this data.

Best,
CK :cow:

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