# adonis, betadiver, and betadisp

**URL:** https://forum.qiime2.org/t/adonis-betadiver-and-betadisp/9364
**Category:** General Discussion
**Created:** [April 24, 2019, 6:12pm UTC](https://forum.qiime2.org/t/adonis-betadiver-and-betadisp/9364 "2019-04-24T18:12:02Z")
**Posts on this page:** 1
**Showing post:** 3

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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [April 25, 2019, 11:53am UTC](https://forum.qiime2.org/t/adonis-betadiver-and-betadisp/9364/3 "2019-04-25T11:53:04Z")

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Just want to chime in to answer some specific questions tossed my way:

> [@devonorourke](#):
>
> Pardon my complete ignorance, but could I have generated this ordination in QIIME anyway?

The ordination yes, the centroids no.

> [@devonorourke](#):
>
> I was worried that the proportion of variance explained by the two eigenvectors was quite low, and wondered how researchers use this information in their interpretations of the figure itself.

It is very low. A small % of variation is explained by the first 2 PCs! Not necessarily a problem, just that season effects (while the effects evident in that plot) are not earth-shatteringly impactful. Makes sense — while you get species turnover by season, many of the same species will be present across proximal seasons.

> [@devonorourke](#):
>
> does the `permdisp` function work for your QIIME Adonis plugin as well as `beta diversity` ?

Yes. Both permdisp and betadisper accept a single "grouping" factor so you just need to test each factor individually if you are comparing to, e.g., a multi-factor adonis test. I think the original paper on the permdisp method explains why multi-factor permdisp is not possible but cannot recall the reason right now...

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