We recently sequenced the rbcl and its2 region from a sample containing only a single known plant species. The reason being is that we would like to add this species to existing databases for future projects. I have a rescript database (qiime2-2022.2) for both rbcl and its2 regions to which we want to add the sequence and taxonomy info. What is the most efficient manner/commands to add a single sequence and species to the databases prior to rerunning "qiime rescript evaluate-fit-classifier"? I have the seqs and taxa.qza's for both databases of interest, rbcl and its2.
Thank you for using RESCRIPt!
All you need to do, is make sure your sequence and taxonomy formatting is identical to your existing RESCRIPt rcbl / its2 databases and then simply run the following
merge commands on each database, after you import the data. Here is an example using rbcl:
qiime feature-table merge-taxa \ --i-data rbcl-taxa.qza new-rbcl-taxa.qza \ --o-merged-data rbcl-taxa-ver02.qza qiime feature-table merge-seqs \ --i-data rbcl-seqs.qza new-rbcl-seqs.qza\ --o-merged-data rbcl-seqs-ver02.qza
From here you can simply re-train your classifier etc...
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