i hope I didn’t missed any topic about it, but I couldn’t find an answer:
i have both Forward and Reverse fasta files of my samples. Is there a way, when demtiliplexing
the reads, to merge the F and R files and add a fixed number of ‘N’ between them?
i’ll elaborate- I did an amplicon sequencing for a 700bp fragment. However my F and R reads are around 280-300 each bp each, so I want to merge them and add 100 'N’s between the R and F.
Is that possible using qiime2?