A question about the “Atacama soil microbiome” tutorial

I am learning QIIME 2 using the Atacama soil microbiome tutorial (https://docs.qiime2.org/2018.8/tutorials/atacama-soils/)

I encountered a problem when I run pair-ends analysis. The tutorial says:

To analyze these data, the sequences that you just downloaded must first be imported into an artifact of type EMPPairedEndSequences.

qiime tools import _
_ --type EMPPairedEndSequences _
_ --input-path emp-paired-end-sequences _
_ --output-path emp-paired-end-sequences.qza

However, I got an errors like this “Invalid value for “–input-path”: Path “emp-paired-end-sequences” does not exist.”

I am not sure what is option “EMPPairedEndSequences” is. It seems there is no such a type option.

Thanks

Hey @sdpapet,

It sounds like you might have missed this step which creates that directory (followed by some download steps).

That is telling QIIME 2 what the data represents. We call it a Semantic Type. You’ll see them a lot in QIIME 2 (for example FeatureTable[Frequency] or SampleData[AlphaDiversity]), that particular type isn’t super interesting, but it is necessary.

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