A question about paired-end sequences analysis

When I did the initial processing steps of paired-end read analysis, I have no way to demultiplex sequences from my library because my sequences contains double-end barcodes (forward read has a barcode, and reverse has one). I refered to the “Atacama soil microbiome” tutorial, and found the demo sequences contain single-end barcode. I have no idea to finish it. Please give me some advice.Thanks a lot!

I want to know this update about the dual-indexing strategiesbe of QIIME2 could be carried out?I just met the problems like that. Thanks.

Hey there @liucong2018!

Unfortunately we don’t yet support directly demuxing dual-index barcodes (open issue). If your reads were generated using an Illumina sequencing platform, I would suggest using their demux tool for this.

Keep us posted! :qiime2: :t_rex:

1 Like

This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.