Hello everyone, I am cofused when I analyzed my16s rDNA of soil sample, I was confused that whichTaxonomy classifiers should I choose, and the primer is 341F and 806R.
I have seen the tutorial of the qiime2, and there has many Taxonomy classifiers of 16s rRNA, but I don't know whether I can use them.
Thanks for your attention
Hi @ChenJin,
You have a couple of options.
- You can simply use the full length SILVA classifier found here.
- You can use RESCRIPt plugin to make your own SILVA classifier as outlined here.
- Some forum members have already made and shared their own versions of the 341F-806R SILVA classifiers. If you trust those, you can likely make use of them.
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Thanks for your answer, it will be very helpful for me.
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