I previously excuted the following command succeasfully for 3 samples:
qiime feature-classifier classify-sklearn --i-classifier gg-13-8-99-515-806-nb-classifier.qza --i-reads /home/temp/Desktop/sequences.qza --o-classification taxonomy.qza`
However now when i am excuting it for more samples i keep getting this error
(1/1) Invalid value for '--i-reads': 'sequences.qza' is not a QIIME 2 Artifact (.qza)
I have tried changing directory, still same error
I placed the file in the working directory, checked if the file is there by giving "ls" command. the sequence.qza file is there in the directory, still the error
i have checked the memory:
I have removed any similar name files from the directory. still no help
Can you run
qiime tools validate on that artifact and screenshot the output?
Hello, Please find below the out put.
(qiime2-2023.7) temp@temp-B365M-DS3H:~$ qiime tools validate sequences.qza
Result sequences.qza appears to be valid at level=max.
hmmm that is weird
I believe this is sometimes caused by a issue in the QIIME2 installation. Could you remove your env and reinstall. After you have a fresh QIIME2 installation, try running this command again
Hello, I just downloaded another classifier and it simply worked.Thank you for your help and time
qiime feature-classifier classify-sklearn
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