General Discussion

Number of basepair to trim in DADA2? (3)
Application of qiime2 in undergraduate education (3)
Are there alternatives to rarefying for alpha diversity estimation? (6)
Importing multiplexed paired end fastq data with barcodes in separate file as text format (15)
Filtering OTU table by abundance <0.1% (7)
QIIME2 community recommended archaea 16S V3V4 primers? (1)
Classifier training parameters ( 2 ) (24)
Truncate reads before VSEARCH with pyrosequecing/ion torrent data (6)
Mycobiome tools (3)
Beta diversity analysis (3)
Combining data after assigning taxa for a meta-analysis (2)
Questions about rarefaction (1)
3 Negative Controls - What is the best way to filter my samples and not delete important data from them (2)
Core Metrics diversity analysis on taxonomic subsets of features (6)
Beta diversity significance values not as expected (3)
Publishable data from Qiime2 (6)
What to do when your expecting expected sequences you don't have (12)
How to trim and quality filter ion torrent data? (7)
CPU usage on cluster machine (4)
How to find the appropriate value of expected errors(--p-max-ee)? (2)
Sir, there's no fly in my digital soup. what gives? ( 2 ) (24)
SILVA: why is chloroplast defined as bacteria? (4)
Build up or tear down: choosing how to represent a sampling unit (2)
SILVA taxonomy naming conventions (3)
How to calculate adjusted P-value for a null hypothesis (10)
Do you guys still remove singletons or doubletons these days (5)
Trim paired sequences with Dada2 drastically reduced the sequence count in Table.qzv? Can anyone help me? (5)
Trimmomatic instead of truncating bases in dada2? (13)
Filtering for private ASVs (11)
Pairwise comparison between samples (6)